| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Mussard, Luke | JR | Dobyns Bennett High School | 1:56.46 | Verified |
| 2 | Quackenbush, Alexander | SR | Daniel Boone High School | 1:58.71 | Verified |
| 3 | Hagood, Caleb | JR | Dobyns Bennett High School | 2:03.30 | Verified |
| 4 | Borghetti-Metz, Roman | SO | Volunteer High School | 2:03.99 | Verified |
| 5 | Cline, Samuel | SR | Daniel Boone High School | 2:07.26 | Verified |
| 6 | Lewis, Bryson | SR | Daniel Boone High School | 2:07.66 | Verified |
| 7 | Sheesley, Ashton | SR | Daniel Boone High School | 2:13.12 | Verified |
| 8 | Roberts, Conner | SR | Daniel Boone High School | 2:13.47 | Verified |
| 9 | Papas, Andreas | JR | University School of Johnson Cit | 2:16.59 | Verified |
| 10 | McCurry, Kevin | 9 | Volunteer High School | 2:16.80 | Verified |
| 11 | Gouge, Elijah | SR | Daniel Boone High School | 2:18.07 | Verified |
| 12 | Falin, Cody | JR | West Ridge High School | 2:18.72 | Verified |
| 13 | Hackney, William | SR | David Crockett High School | 2:22.85 | Verified |
| 14 | Shaffer, Brandon | SR | West Ridge High School | 2:23.28 | Verified |
| 15 | Nemier, Shane | JR | Science Hill High School | 2:24.96 | Verified |
| 16 | Ailshie, Eamon | 9 | Dobyns Bennett High School | 2:25.00 | Unverified: |
| 17 | Osborne, Hayden | JR | University School of Johnson Cit | 2:26.62 | Verified |
| 18 | Whaley, Andrew | JR | Daniel Boone High School | 2:27.09 | Verified |
| 19 | Haas, Reid | JR | West Ridge High School | 2:28.50 | Verified |
| 20 | Wellman, Ethan | SR | Dobyns Bennett High School | 2:28.68 | Verified |
| 21 | Parks, Noah | SO | Dobyns Bennett High School | 2:29.06 | Verified |
| 22 | Mussard, Isaac | 9 | University School of Johnson Cit | 2:31.60 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Gallati, Paul | 9 | Science Hill High School | 2:32.61 | Verified |
| 2 | Livesay, Isaiah | JR | Volunteer High School | 2:32.89 | Verified |
| 3 | Bishop, Drew | JR | Dobyns Bennett High School | 2:33.50 | Verified |
| 4 | Lively, William | SO | Dobyns Bennett High School | 2:35.42 | Verified |
| 5 | Dorsey, Mason | 9 | Daniel Boone High School | 2:42.25 | Verified |
| 6 | Burns, Connor | SO | Volunteer High School | 2:49.20 | Verified |
| 7 | Hathaway, Joshua | SO | Volunteer High School | 2:53.95 | Verified |
| 8 | Musser, Luke | SO | Dobyns Bennett High School | 3:01.67 | Verified |
| 9 | Ard, Nicholas | 9 | Daniel Boone High School | NT | Unverified: |
| 10 | Battel, Fisher | 9 | Daniel Boone High School | NT | Unverified: |
| 11 | Clare, Sean | SR | West Ridge High School | NT | Unverified: |
| 12 | Culp, Ben | 9 | Dobyns Bennett High School | NT | Unverified: |
| 13 | Davis, Casen | JR | David Crockett High School | NT | Unverified: |
| 14 | Emmette, Drew | 9 | Dobyns Bennett High School | NT | Unverified: |
| 15 | Gallati, Ryan | 9 | Science Hill High School | NT | Unverified: |
| 16 | Hensley, Aaron | 9 | Dobyns Bennett High School | NT | Unverified: |
| 17 | Krell, Luke | SR | David Crockett High School | NT | Unverified: |
| 18 | Parsons, Eli | 9 | David Crockett High School | NT | Unverified: |
| 19 | Reyes, Isiah | 9 | West Ridge High School | NT | Unverified: |
| 20 | Sheesley, Landon | 9 | Daniel Boone High School | NT | Unverified: |
| 21 | Thompson, Colton | SO | Daniel Boone High School | NT | Unverified: |
| 22 | Verble, Zach | SO | David Crockett High School | NT | Unverified: |