| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | ALLEN, GRACIE | Dobyns Bennett High School | 2:23.36 |
| 2 | CORDELL, MORGAN | Dobyns Bennett High School | 2:26.97 |
| 3 | RUSSUM, EMMA | Dobyns Bennett High School | 2:27.98 |
| 4 | MCREYNOLDS, JULIANNA | Dobyns Bennett High School | 2:29.28 |
| 5 | PICKUP, MACEE | Dobyns Bennett High School | 2:31.41 |
| 6 | DULANEY, RACHEL | David Crockett High School | 2:34.32 |
| 7 | FLYNN, KATHERINE | Science Hill High School | 2:36.31 |
| 8 | KORODY, MCKENNA | Morristown-West High School | 2:37.55 |
| 9 | MCKINNEY, ELISE | Volunteer High School | 2:37.73 |
| 10 | ROY, TAYLOR | David Crockett High School | 2:38.76 |
| 11 | MCCOY, MARLEY | Daniel Boone High School | 2:38.85 |
| 12 | WADE, CHLOE | Sevier Co. High School | 2:40.88 |
| 13 | FLORES, SANDRA | Morristown-East High School | 2:42.05 |
| 14 | HAAS, ASHLYN | Morristown-West High School | 2:42.71 |
| 15 | BARNES, MEGAN | Science Hill High School | 2:43.78 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MULHOLLEN, JULIA | Greeneville High School | 2:44.26 |
| 2 | GUINN, KAYLEE | Jefferson County High School | 2:44.53 |
| 3 | HULSE, EMMALINE | David Crockett High School | 2:44.87 |
| 4 | BORISOV, NELLY | Jefferson County High School | 2:46.96 |
| 5 | WILMOTH, SYDNEY | Volunteer High School | 2:48.50 |
| 6 | MCNALLY, CELINE | Volunteer High School | 2:49.05 |
| 7 | BOWERY, GRACE | Sullivan South High School | 2:51.03 |
| 8 | DAY, KIRSTEN | University School of Johnson Cit | 2:51.07 |
| 9 | WALLEN, JOCELYN | Greeneville High School | 2:53.95 |
| 10 | FELLERS, NATALIE | Daniel Boone High School | 2:54.53 |
| 11 | MONDAY, CHESNEY | Carter High School | 2:54.77 |
| 12 | KELLS, HALEY | West Greene High School | 2:56.27 |
| 13 | ZAPATA, JENNIFER | Jefferson County High School | 2:57.43 |
| 14 | FARMER, LINDSAY | Morristown-West High School | NT |
| 15 | KING, ABBEY | West Greene High School | 2:59.38 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | SIMERLY, TATIANA | Happy Valley High School | 2:59.82 |
| 2 | WALLS, MARINDA | Daniel Boone High School | 2:59.86 |
| 3 | WARD, EMILY | David Crockett High School | 3:00.27 |
| 4 | ARNEY, KAYLEE | Abingdon High School | 3:01.23 |
| 5 | RUDOLPH, JOSIE | Jefferson County High School | 3:01.49 |
| 6 | SUMMEY, MELINA | University School of Johnson Cit | 3:02.61 |
| 7 | SEGURA CUBELLS, IRENE | Sullivan South High School | 3:05.67 |
| 8 | DAVIDSON, AUBREY | University School of Johnson Cit | 3:07.68 |
| 9 | WOLFE, HANNA | Sullivan South High School | 3:10.35 |
| 10 | ROARK, KALEE | Happy Valley High School | 3:11.33 |
| 11 | ALBRO, SYDNEY | Abingdon High School | 3:15.00 |
| 12 | ARNOLD, CHELSEA | Happy Valley High School | 3:26.16 |
| 13 | CARPENTER, MADDY | Happy Valley High School | 3:28.54 |
| 14 | CATHCART, SOPHIA | Marion | NT |
| 15 | GRIFFITH, NICOLE | Daniel Boone High School | NT |
| 16 | JONES, MEGAN | West Greene High School | NT |
| 17 | MCNEW, ISABELLA | Volunteer High School | NT |