| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | West Greene High School | A | DNS |
| 2 | Sullivan South High School | A | 54.59 |
| 3 | Morristown-East High School | A | 53.47 |
| 4 | Science Hill High School | A | 53.41 |
| 5 | Carter High School | A | 51.39 |
| 6 | Volunteer High School | A | 52.65 |
| 7 | Science Hill High School | B | 53.41 |
| 8 | Jefferson County High School | A | 54.34 |
| 9 | Daniel Boone High School | A | 55.56 |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 2 | University School of Johnson Cit | A | NT |
| 3 | Elizabethton High School | A | 1:02.20 |
| 4 | Jefferson County High School | B | 59.00 |
| 5 | Volunteer High School | B | 56.00 |
| 6 | David Crockett High School | A | 56.39 |
| 7 | Daniel Boone High School | B | 59.56 |
| 8 | Greeneville High School | A | DNS |
| 9 | Happy Valley High School | A | NT |