| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | David Crockett High School | A | 4:00.21 |
| 2 | Jefferson County High School | A | 3:52.14 |
| 3 | Morristown-West High School | A | 3:41.73 |
| 4 | Sevier Co. High School | A | 3:36.45 |
| 5 | Science Hill High School | A | 3:32.64 |
| 6 | Dobyns Bennett High School | A | 3:34.50 |
| 7 | Daniel Boone High School | A | 3:36.96 |
| 8 | Morristown-East High School | A | 3:45.44 |
| 9 | Volunteer High School | A | 3:58.07 |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 2 | Abingdon High School | A | NT |
| 3 | Greeneville High School | A | 4:08.34 |
| 4 | Morristown-West High School | B | DNS |
| 5 | Jefferson County High School | B | 3:52.14 |
| 6 | Daniel Boone High School | B | 3:56.96 |
| 7 | Science Hill High School | B | DNS |
| 8 | Elizabethton High School | A | 4:32.73 |
| 9 | University School of Johnson Cit | A | NT |