| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | NEWSOME, NATALIE | Happy Valley High School | 50.82 |
| 2 | BARRETT, BLAIRE | Dobyns Bennett High School | 50.53 |
| 3 | RICKETTS, MADISON | Dobyns Bennett High School | 50.03 |
| 4 | EPPARD, EMILY | Greeneville High School | 49.52 |
| 5 | MCELHANEY, AISLINN | Sevier Co. High School | 46.13 |
| 6 | TOLLIVER, EVA | Carter High School | 49.03 |
| 7 | MAYS, EMILY | Abingdon High School | 49.66 |
| 8 | TAYLOR, LINDSEY | Science Hill High School | 50.34 |
| 9 | SCOTT, HALLE | David Crockett High School | 50.79 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | SOOKLAL, ASHLEY | Abingdon High School | 1:02.76 |
| 2 | CASTELLANOS, DEBORAH | Morristown-West High School | NT |
| 3 | STROUD, BAYLEE | Morristown-West High School | 56.07 |
| 4 | WILSON, KATHLEEN | Science Hill High School | 52.83 |
| 5 | WATTS, KRISTA | Jefferson County High School | 50.98 |
| 6 | CHRISTIAN, EMILY | Volunteer High School | 52.11 |
| 7 | KING, ABBEY | West Greene High School | 55.62 |
| 8 | BROWN, CHLOE | Daniel Boone High School | 56.25 |
| 9 | DALTON, TAMRA | Morristown-West High School | 56.74 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 4 | JNO-BAPTISTE, CHENOA | West Greene High School | NT |
| 5 | KEYS, ERIN | David Crockett High School | 1:03.43 |
| 6 | PARTIN, CRYSTAL | Morristown-West High School | 1:09.62 |