| Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|
| 1 | JACKSON, Makayla | SR | Claflin | 1:00.96 |
| 2 | PUENTE, Eskolunbe | SR | Grand Valley St. | 1:01.50 |
| 3 | WITMER, Mary | SR | Liberty | 1:01.88 |
| 4 | ETANISLAO, Klerianne | JR | Liberty | 1:02.00 |
| 5 | POLSGROVE, Kylie | SO | Liberty | 1:02.77 |
| 6 | ESPINOZA, Alyssa | SR | North Dakota | 1:04.21 |
| 7 | ARDLEY, Shantel | FR | Charleston Southern | 1:04.91 |
| 8 | LINN, Katherine | JR | Col. of Charleston | 1:05.00 |
| 9 | DUPREE, Emma | FR | Col. of Charleston | 1:05.63 |
| 10 | DRIVER, Holly | FR | Col. of Charleston | 1:05.70 |
| 11 | ANDERSON, Aleksandra | FR | North Dakota | 1:06.11 |
| 12 | LITTLE, Destinie | FR | Charleston Southern | 1:06.82 |
| 13 | FRAZIER, Areale | FR | Charleston Southern | 1:08.14 |
| 14 | ORY, Sara | Bowdoin | 1:08.52 | |
| 15 | ENNIS, Kayla | FR | Col. of Charleston | 1:09.80 |
| 16 | MASSIE, Nina | FR | Charleston Southern | 1:10.06 |
| 17 | SAMUELS, Jay'Kayla | FR | Westminster (Pa.) | 1:10.99 |
| 18 | IRELAND, Keilah | SR | Westminster (Pa.) | 1:11.00 |
| 19 | KRAUSE, Clarece | FR | Methodist | 1:12.15 |
| 20 | BADAMO, Taylor | FR | Cuyahoga CC | 1:18.00 |
| 21 | FRENCH, Natania | FR | Cuyahoga CC | 1:21.00 |
| 22 | BARLE, Marissa | FR | Cuyahoga CC | 1:28.00 |
| 23 | MORRIS, Rayauna | FR | Cuyahoga CC | 1:30.00 |
| 24 | MORRISON, Tametris | JR | Claflin | NT |
| 25 | MCKIE, Faith | FR | Claflin | NT |
| 26 | HIGGINS, Julia | SO | Wooster | NT |
| 27 | O'CONNELL, Jessica | JR | Grand Valley St. | NT |
| 28 | LUBA, Breanna | JR | Grand Valley St. | NT |