| Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|
| 1 | BURNETT, Danielle | SR | Charleston Southern | 54.52 |
| 2 | CLARKE, Stephanie | SR | North Dakota | 55.81 |
| 3 | COLEMAN, Kolisha | SR | Charleston Southern | 55.92 |
| 4 | ASHE, Payton | JR | South Carolina St. | 56.35 |
| 5 | SHAW, Samantha | SO | Northern Kentucky | 56.85 |
| 6 | CALLOWAY, Faith | SO | USC-Beaufort | 57.12 |
| 7 | KEYS, BriAuna | SO | Northern Kentucky | 57.29 |
| 8 | COOPER, Maiya | JR | USC-Beaufort | 57.36 |
| 9 | ADAMS, Alexis | SO | Charleston Southern | 57.98 |
| 10 | JOHNSON, Sarah | SR | Citadel | 58.96 |
| 11 | LITTLE, Emani | SR | Citadel | 59.59 |
| 12 | FRANCIS, Ishawn | JR | Claflin | 59.76 |
| 13 | MUTCHERSON, Cort'Nasha | FR | USC-Beaufort | 59.80 |
| 14 | ANDREWS, Naleta | FR | Cuyahoga CC | 1:00.00 |
| 15 | GRAVELLE, Jordan | JR | Col. of Charleston | 1:00.00 |
| 16 | SHIPLEY, Caroline | Bowdoin | 1:01.11 | |
| 17 | TAYLOR, Brandi | SR | Claflin | 1:01.34 |
| 18 | LEWIS, Brianna | SO | Col. of Charleston | 1:01.92 |
| 19 | BANKS, Aarionna | SO | Cuyahoga CC | 1:02.00 |
| 20 | THOMPSON, Summer | SO | Citadel | 1:02.10 |
| 21 | MURRAY, Hannah | SO | Citadel | 1:02.52 |
| 22 | GOWER, Amanda | FR | Bethany (W.V.) | 1:03.32 |
| 23 | SANCHEZ, Zaidybelle | FR | Methodist | 1:03.65 |
| 24 | PEGUES, Daija | SO | Claflin | 1:04.31 |
| 25 | PRITCHARD, Lindsey | FR | Methodist | 1:04.50 |
| 26 | WESLEY, Zakiyah | FR | Cuyahoga CC | 1:04.75 |
| 27 | STEFFEN, Gabriella | FR | Charleston Southern | 1:06.50 |
| 28 | MOORE, Quiona | SO | Methodist | 1:07.00 |
| 29 | WALKER, Naomi | FR | Cuyahoga CC | 1:09.00 |