| Lane | Team | Squad | Seed |
|---|---|---|---|
| 3 | James Island | A | NT |
| 4 | Burke | A | 4:42.09 |
| 5 | Baptist Hill | A | NT |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | Stall | A | 4:01.39 |
| 2 | Academic Magnet | A | 3:52.21 |
| 3 | St. Johns | A | 3:47.14 |
| 4 | Wando | A | 3:31.35 |
| 5 | Lucy Beckham | A | 3:35.07 |
| 6 | West Ashley | A | 3:50.15 |
| 7 | North Charleston | A | 4:00.26 |
| 8 | Military Magnet Academy | A | 4:23.54 |