| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | HARLESS, Andre | SO | Volunteer High School | 43.50 |
| 2 | MORGAN, Connor | JR | Science Hill High School | 43.15 |
| 3 | TESNEAR, Tyler | 9 | Dobyns Bennett High School | 42.16 |
| 4 | FARIS, Austin | SR | Patrick Henry (G. Spring) | 40.69 |
| 5 | LANE, Austin | SR | John I. Burton | 41.60 |
| 6 | WARD, Ethan | SR | Sullivan South High School | 42.35 |
| 7 | JENKINS, Avery | JR | Union High School | 43.39 |
| 8 | GENCO, Gunner | SO | Ridgeview High School | 43.60 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 3 | MICHALIK, Nathan | 9 | Volunteer High School | 44.47 |
| 4 | SINK, Grayson | JR | Abingdon High School | 43.74 |
| 5 | WARD, Jack | SR | Castlewood | 44.38 |
| 6 | POLIER, Mason | SO | Union High School | 44.75 |