| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Wando | A | 47.35 | ||||
| 3 | Bishop England | A | Bryant | Hale | Mahoney | Seitzinger | 45.46 |
| 4 | Philip Simmons | A | Hirschman | Jenkins | Williams | Tonon | 43.82 |
| 5 | Bishop England | B | Tregner | Keith | Matsik | Ridenour | 45.46 |
| 6 | Wando | B | 47.35 | ||||
| 7 | Palmetto Christian Academy | A | Newbern | Parrish | Rodenberg | Pollard | NT |