| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WELLS, Julia | Tuscola | 5:30.08 |
| 2 | PENDERGRASS, Breanna | Franklin | 5:44.53 |
| 3 | MILLER, Ellie | Smoky Mountain | 5:49.00 |
| 4 | POPE, Abigail | Franklin | 6:00.97 |
| 5 | MERAZ-QUIJADA, Julianna | Swain County | 6:08.08 |
| 6 | RADECKI, Molly | Franklin | 6:30.00 |
| 7 | PLEMMONS, Cheyenne | Franklin | 7:00.00 |
| 8 | RICH, Isabella | Pisgah | 8:00.50 |
| 9 | CASTELLANO-MARTINEZ, Carola | Swain County | NT |
| 10 | GLOVER, Berklee | Rosman | NT |
| 11 | KEEL-AGUILERA, Kyla | Cherokee | NT |
| 12 | KETTLER, Zoe | Smoky Mountain | NT |
| 13 | LANCE, Regan | Pisgah | NT |
| 14 | LANNING, Autumn | Pisgah | NT |
| 15 | LOSSIAH, Maya | Cherokee | NT |
| 16 | MARROQUIN, Karla | North Henderson | NT |
| 17 | SIAN, Darlin | Pisgah | NT |
| 18 | ZAMUDIO, Anahi | Pisgah | NT |