| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CHAPMAN, Taylor | Tri-Cities Christian Academy | 2:49.98 |
| 2 | TOLLEY, Haleigh | Tri-Cities Christian Academy | 2:50.59 |
| 3 | SCOTT, Cadence | Virginia Bearcats | 2:51.57 |
| 4 | HUMPHREY, Lindsey | Jefferson County High School | 2:52.96 |
| 5 | ARENAS GONZALES, Karen | Morristown-West High School | 2:53.49 |
| 6 | VELEDIAZ, Maria | Jefferson County High School | 3:00.00 |
| 7 | MENGLE, Jocelyn | Jefferson County High School | 3:01.83 |
| 8 | GUERRERO, Leslie | Morristown-East High School | 3:08.84 |
| 9 | HOGSTON, Emma | Virginia Bearcats | 3:11.09 |
| 10 | GWINN, Madison | Chilhowie | 3:19.88 |
| 11 | GONZALEZ, Aubrey | Chilhowie | 3:31.88 |
| 12 | MURPH, Jazzmine | Virginia Bearcats | NT |
| 13 | RICHMOND, Ruby | Daniel Boone High School | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | PAPAS, Kalli | University School of Johnson City | 2:38.99 |
| 2 | GROSS, Kendyl | Chilhowie | 2:39.29 |
| 3 | HACKETT, Jane | Elizabethton High School | 2:39.63 |
| 4 | CLEMENT, Ava | Science Hill High School | 2:40.03 |
| 5 | ISAACS, Madeline | Elizabethton High School | 2:40.29 |
| 6 | MCKINNON, Cambria | Tennessee High School | 2:41.38 |
| 7 | KOCHEMBA, Raya | Volunteer High School | 2:41.68 |
| 8 | COUCH, Kennedie | Morristown-West High School | 2:41.98 |
| 9 | HATLEY, Adria | Science Hill High School | 2:44.53 |
| 10 | HIXSON, Lilly | David Crockett High School | 2:45.12 |
| 11 | TOWNSEND, Ashlyn | West Ridge High School | 2:47.82 |
| 12 | WATSON, Kendall | West Ridge High School | 2:48.25 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | TUTTEROW, Lily | Science Hill High School | 2:22.02 |
| 2 | MCGEE, Maesyn | David Crockett High School | 2:24.06 |
| 3 | COWARD, Elizabeth | Cherokee High School | 2:27.97 |
| 4 | DEAL, Kenady | Cherokee High School | 2:29.81 |
| 5 | MOORE, Kiley | David Crockett High School | 2:30.82 |
| 6 | PIAZZA, Savannah | David Crockett High School | 2:32.90 |
| 7 | MALAVOLTI, Alana | George Wythe (Wytheville) | 2:32.98 |
| 8 | BARNES, Payton | Morristown-West High School | 2:33.35 |
| 9 | WRIGHT, Sydney | Daniel Boone High School | 2:34.86 |
| 10 | LOUTER, Elizabeth | Daniel Boone High School | 2:36.03 |
| 11 | JOHNSON, Emma | University School of Johnson City | 2:36.48 |
| 12 | BANDY, Aniyah | Cherokee High School | 2:38.15 |