| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Timberland | A | 3:57.39 | Stratford Home Meet #2 |
| 3 | Woodland | A | 3:41.79 | Tri County Meet of Champions |
| 4 | Philip Simmons | A | 3:34.10 | Berkeley County School District |
| 5 | Burke | A | 3:36.51 | Cane Bay Home Meet #2 (Senior Ni |
| 6 | Academic Magnet | A | 3:50.68 | Charleston County School Distric |
| 7 | Andrews High School | A | DNS | Carvers Bay High 7th, 8th and 9t |