| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Academic Magnet | A | 4:31.92 | Charleston County School Distric |
| 3 | Andrews High School | A | 4:25.02 | Georgetown County Quad Champions |
| 4 | Philip Simmons | A | 4:13.02 | Berkeley County School District |
| 5 | Woodland | A | 4:15.86 | Patriot Invitational |
| 6 | Timberland | A | 4:27.85 | Cane Bay Track and Field Invitat |