| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 146 | Kelley, Adam | Daniel Boone High School | 4:19.00 |
| 2 | 186 | McCurry, Kevin | Volunteer High School | 4:23.24 |
| 3 | 366 | Harker, Harrison | University School of Johnson City | 4:23.73 |
| 4 | 180 | Jennings, Isaac | Volunteer High School | 4:35.04 |
| 5 | 176 | Robinson, Dylan | Daniel Boone High School | 4:40.70 |
| 6 | 139 | Marciante, Seth | Daniel Boone High School | 4:44.14 |
| 7 | 72 | Tankersley, Spencer | Dobyns Bennett High School | 4:45.33 |
| 8 | 364 | Mathewes, Lucas | University School of Johnson City | 4:49.15 |
| 9 | 315 | Bahulekar, Aarav | Science Hill High School | 4:51.59 |
| 10 | 205 | Flanary, Sebastian | Volunteer High School | 4:54.50 |
| 11 | 49 | Garner, Luke | Dobyns Bennett High School | 4:55.50 |
| 12 | 170 | Dorsey, Mason | Daniel Boone High School | 4:59.26 |
| 13 | 27 | Armstrong, Cooper | Dobyns Bennett High School | 5:00.64 |
| 14 | 164 | Gouge, Carson | Daniel Boone High School | 5:00.98 |
| 15 | 280 | Burzynski, Joshua | Science Hill High School | 5:02.40 |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 357 | Scheller, Burk | University School of Johnson City | 5:03.24 |
| 2 | 282 | Holden, Camden | Science Hill High School | 5:03.30 |
| 3 | 120 | Comire, Able | Kingsport Area Christian Home Education Association | 5:03.87 |
| 4 | 187 | Trejo, Leonel | Volunteer High School | 5:05.75 |
| 5 | 145 | Cutshall, Sam | Daniel Boone High School | 5:11.88 |
| 6 | 166 | Ashmore, Tanner | Daniel Boone High School | 5:15.00 |
| 7 | 121 | Jones, Uriah | Kingsport Area Christian Home Education Association | 5:16.90 |
| 8 | 80 | Doiron, Noah | Dobyns Bennett High School | 5:18.63 |
| 9 | 188 | Buchanan, Dakotah | Volunteer High School | 5:22.76 |
| 10 | 181 | Boyd, Isaac | Volunteer High School | 5:24.16 |
| 11 | 275 | O'Neil, Zaylen | Science Hill High School | 5:29.86 |
| 12 | 175 | Sturgill, Ayden | Daniel Boone High School | 5:30.00 |
| 13 | 19 | Freeman, Ian | Dobyns Bennett High School | 5:35.43 |
| 14 | 346 | Walden, Trysten | Sullivan East High School | 5:37.15 |
| 15 | 317 | Henderlite, John | Sullivan East High School | 5:37.23 |
| 16 | 200 | Flanery, Jack | Volunteer High School | 5:38.39 |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 130 | Williams, Lucas | Daniel Boone High School | 5:39.37 |
| 2 | 133 | Danko, Hayden | Daniel Boone High School | 5:40.15 |
| 3 | 157 | Deaton, John | Daniel Boone High School | 5:41.23 |
| 4 | 202 | Trejo, Oscar | Volunteer High School | 5:43.53 |
| 5 | 250 | Cox, Aidan | West Ridge High School | 5:44.22 |
| 6 | 126 | Munier, Dawson | Daniel Boone High School | 5:48.35 |
| 7 | 112 | Houser, Caleb | Kingsport Area Christian Home Education Association | 5:56.55 |
| 8 | 286 | Slusser, Luke | Science Hill High School | 5:59.48 |
| 9 | 367 | Adler, Pierce | University School of Johnson City | 6:01.02 |
| 10 | 316 | Cromer, Brayden | Sullivan East High School | 6:03.20 |
| 11 | 217 | Brown, Andrew | West Ridge High School | 6:10.78 |
| 12 | 137 | Wilgocki, Bryson | Daniel Boone High School | 6:12.59 |
| 13 | 323 | Cromer, Ian | Sullivan East High School | 6:12.99 |
| 14 | 110 | Gibbons, Mark | Kingsport Area Christian Home Education Association | NT |
| 15 | 332 | Guy, Briley | Sullivan East High School | NT |
| 16 | 241 | Johnson, Levi | West Ridge High School | NT |