| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 132 | Honeycutt, Sadie | Daniel Boone High School | 2:16.09 |
| 2 | 68 | Turner, Tiesha | Dobyns Bennett High School | 2:17.48 |
| 3 | 52 | Mazzilli, Mattea | Dobyns Bennett High School | 2:19.49 |
| 4 | 155 | Nutter, Ava | Daniel Boone High School | 2:23.98 |
| 5 | 272 | Hunter, Ryann | Science Hill High School | 2:29.66 |
| 6 | 293 | Haywood, Evy | Science Hill High School | 2:32.51 |
| 7 | 123 | Robinson, Hannah | Daniel Boone High School | 2:33.94 |
| 8 | 135 | Louter, Elizabeth | Daniel Boone High School | 2:38.43 |
| 9 | 149 | Wright, Sydney | Daniel Boone High School | 2:40.11 |
| 10 | 13 | Brickey, Emma | Dobyns Bennett High School | 2:41.02 |
| 11 | 311 | Clement, Ava | Science Hill High School | 2:42.06 |
| 12 | 151 | Newell, Rachel | Daniel Boone High School | 2:42.40 |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 330 | Velasquez, Valeria | Sullivan East High School | 2:44.42 |
| 2 | 274 | Hatley, Adria | Science Hill High School | 2:49.85 |
| 3 | 90 | Chapman, Taylor | Tri-Cities Christian Academy | 2:49.98 |
| 4 | 258 | Stubbs, Reagan | West Ridge High School | 2:50.05 |
| 5 | 154 | Richmond, Ruby | Daniel Boone High School | 2:50.65 |
| 6 | 185 | Fleishour, Ava | Volunteer High School | 2:51.89 |
| 7 | 212 | Watson, Kendall | West Ridge High School | 2:51.99 |
| 8 | 328 | Rodriguez-Alvidrez, Lexi | Sullivan East High School | 2:52.32 |
| 9 | 359 | Maylock, Lorenza | University School of Johnson City | 2:52.94 |
| 10 | 365 | Tai, Emily | University School of Johnson City | 2:53.99 |
| 11 | 214 | Greer, Zayda | West Ridge High School | 2:57.21 |
| 12 | 348 | Feathers, Reagan | Sullivan East High School | 2:57.68 |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 22 | McCarty, Juliet | Dobyns Bennett High School | 2:57.77 |
| 2 | 114 | Olinger, Berkeley | Kingsport Area Christian Home Education Association | 3:00.05 |
| 3 | 116 | Gonce, Naomi | Kingsport Area Christian Home Education Association | 3:01.49 |
| 4 | 363 | McKinney, Darcy | University School of Johnson City | 3:02.10 |
| 5 | 95 | Tolley, Haleigh | Tri-Cities Christian Academy | 3:03.54 |
| 6 | 235 | Mullins, Carrera | West Ridge High School | 3:05.07 |
| 7 | 269 | Brooks, Olivia | Science Hill High School | 3:07.60 |
| 8 | 333 | Thompson, Hayden | Sullivan East High School | 3:15.39 |
| 9 | 221 | Glover, Britton | West Ridge High School | 3:36.07 |
| 10 | 78 | Carroll, Lily | Dobyns Bennett High School | NT |
| 11 | 184 | Keck, Dixie | Volunteer High School | NT |
| 12 | 199 | Kochemba, Raya | Volunteer High School | NT |
| 13 | 324 | Purswani, Angelina | Sullivan East High School | NT |