| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 258 | Brown, Emily | David Crockett High School | 59.70 |
| 3 | 19 | Wolfe, Reagan | Dobyns Bennett High School | 56.97 |
| 4 | 142 | Weber, Sophie | Daniel Boone High School | 55.01 |
| 5 | 412 | Williams, Ava | University School of Johnson City | 52.55 |
| 6 | 339 | Emmert, Audrey | Science Hill High School | 54.48 |
| 7 | 148 | Brown, Tyla | Daniel Boone High School | 55.50 |
| 8 | 418 | Stack, Maddie | Unicoi Co. High School | 59.29 |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 90 | Merryman, Addyson | Elizabethton High School | 52.13 |
| 2 | 468 | Brown, Carley | West Ridge High School | 50.04 |
| 3 | 35 | Feldmann, Marley | Dobyns Bennett High School | 49.71 |
| 4 | 184 | Bucaro, Redding | Daniel Boone High School | 48.02 |
| 5 | 244 | Romans, Keaton | Tennessee High School | 44.88 |
| 6 | 44 | Cradic, Eden | Dobyns Bennett High School | 46.53 |
| 7 | 347 | Hale, Caroline | Science Hill High School | 48.79 |
| 8 | 205 | Harrison, Elizabeth | Tennessee High School | 49.71 |
| 9 | 323 | Webb, Lyndie | Science Hill High School | 50.20 |