| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Grandview Middle School | A | 2:07.31 | Washington County Championship |
| 3 | Gray Middle School | A | 2:00.40 | Washington County Middle School |
| 4 | Surgoinsville Middle School | A | 2:00.72 | Sullivan East Middle #3 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Jonesborough Middle School | A | 1:55.58 | Washington County Championship |
| 2 | Pigeon Forge Junior High | A | 1:48.44 | Sevier County MS Meet #2 |
| 3 | Happy Valley Middle School | A | 1:46.11 | Liberty Bell Invitational |
| 4 | Ridgeview Middle / Elementary School | A | 1:47.80 | Washington County Middle School |
| 5 | Rogersville Middle School | A | 1:52.97 | Mobuck Middle School Invitationa |
| 6 | South Greene Middle School | A | 1:55.74 | 2025 Patriot Invitational |