| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Surgoinsville Middle School | A | 1:04.73 | Pioneer Invitational Meet 1 |
| 3 | Ridgeview Middle / Elementary School | A | 1:01.85 | 2025 Patriot Invitational |
| 4 | Jonesborough Middle School | A | 1:03.66 | Washington County Championship |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | WestView Middle School (limestone) | A | 1:01.10 | Washington County Middle School |
| 2 | Boones Creek Middle School | A | 1:00.35 | Washington County Championship |
| 3 | Happy Valley Middle School | A | 59.39 | Sullivan East Middle #2 |
| 4 | Grandview Middle School | A | 1:00.06 | Mobuck Middle School Invitationa |
| 5 | Lamar Middle School | A | 1:00.36 | Pioneer Invitational Meet 1 |
| 6 | Unicoi Co. Middle School | A | 1:01.62 | Sullivan East Middle #5 (Invite |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Rogersville City School | A | 58.76 | Mobuck Middle School Invitationa |
| 2 | Gatlinburg-Pittman Middle School | A | 58.17 | Sevier County MS Meet #2 |
| 3 | Gray Middle School | A | 57.98 | Washington County Championship |
| 4 | South Greene Middle School | A | 58.12 | Pioneer Invitational Meet 1 |
| 5 | Pigeon Forge Junior High | A | 58.34 | Pigeon Forge JR High Meet 2 |
| 6 | Rogersville Middle School | A | 59.25 | Pioneer Invitational Meet 1 |