| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Murphy | A | DNS | Murphy High School Conference Me |
| 3 | Swain County | A | 4:33.10 | Swain County High School Invitat |
| 4 | Cherokee | A | 4:29.20 | Robbinsville Meet #1SMC |
| 5 | Hayesville | A | 4:31.48 | Murphy High School Conference Me |
| 6 | Andrews | A | 4:42.38 | Andrews High School Home Meet |
| 7 | Tri-County Early College | A | 5:16.87 | Hayesville High Home Meet |