| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Jonesborough Middle School | A | 1:07.31 | Sullivan East Middle #3 |
| 3 | Surgoinsville Middle School | A | 1:04.73 | Pioneer Invitational Meet 1 |
| 4 | Rogersville City School | A | 1:05.41 | GMS Home Meet #1 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lakeway Christian Academy | A | 1:02.35 | CAK-KYA Track Classic |
| 2 | East Ridge MS Whitesburg | A | 1:01.25 | |
| 3 | Lincoln Heights Middle School | A | 1:00.23 | MMS-LH & MV |
| 4 | Grandview Middle School | A | 1:01.10 | 2025 Patriot Invitational |
| 5 | Boones Creek Middle School | A | DNS | Pioneer Invitational Meet 1 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Rogersville Middle School | A | 59.25 | Pioneer Invitational Meet 1 |
| 2 | John Sevier Middle School | A | 56.63 | Burger Bar MS Invitational |
| 3 | Robinson Middle School | A | 53.27 | Burger Bar MS Invitational |
| 4 | Greeneville Middle School | A | 54.18 | Maryville MS Meet #2 |
| 5 | Church Hill Middle School | A | 58.67 | Sullivan East Middle #3 |
| 6 | Maury Middle School | A | 59.28 | MMS-LH & MV |