| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Richlands | A | 47.85 | The 30th Annual Judy Thomas Was |
| 3 | Marion | A | 46.53 | The Polished Diamond Spa Rebel L |
| 4 | Carroll County | A | 46.94 | Blue Demon Invitational |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Pulaski County | A | DNF | Blue Demon Invitational |
| 3 | Abingdon High School | A | 43.51 | Blue Demon Invitational |
| 4 | Lee | A | 43.64 | Morgan-McClure Invitational |
| 5 | Union High School | A | 44.78 | Southwest Insurance Relays |