| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Bennett, La'Mya | Virginia Bearcats | 1:19.20 | Rebel Last Chance Meet |
| 2 | Scott, Cadence | Virginia Bearcats | 1:11.37 | Wamplers Insurance Middle School |
| 3 | Laws, Alyssa | Lee | 1:10.78 | Lee Last Chance |
| 4 | Cowden, Leah | Lee | 1:11.02 | |
| 5 | Livesay, Marissa | Lee | 1:13.45 | |
| 6 | Williams, Lila | Graham | 1:25.89 | Food City Track Classic |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Rose, Megan | Graham | 1:10.26 | Elementary/MS/HS Invitational |
| 2 | Foutz, Laken | Marion | 1:09.48 | The 30th Annual Judy Thomas Was |
| 3 | Stewart, Elyanna | Abingdon High School | 1:04.50 | Mountain 7 District Championship |
| 4 | Robinson, Savannah | Virginia Bearcats | 1:07.01 | Wampler's Insurance Abingdon Inv |
| 5 | Barnett, Caleigh | Abingdon High School | 1:09.55 | Blue Demon Invitational |
| 6 | Britton, Julia | Lee | 1:10.67 |