| Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|
| 1 | Welsing, Melanie | SO | Queens (N.C.) |
1:01.11 |
| 2 | Williams, Keyanna | SR | Southern Wesleyan |
1:05.37 |
| 3 | Pernell, Lauren | SO | Anderson (S.C.) |
1:05.71 |
| 4 | Rowe, Nathalia | FR | Brenau |
1:05.88 |
| 5 | Castillo, Isabela | FR | Southern Wesleyan |
1:05.99 |
| 6 | Kramer, Tessa | FR | Converse |
1:07.00 |
| 7 | Cothron, Shelby | FR | Cumberlands |
1:07.00 |
| 8 | Stevens, Cora | JR | Cumberlands |
1:07.13 |
| 9 | Martin, Hailey | JR | Cumberlands |
1:07.57 |
| 10 | Lema, Kayla | FR | Southern Wesleyan |
1:07.80 |
| 11 | Dunlap, Jasmine | FR | Wingate |
1:07.89 |
| 12 | Djokic, Lidija | FR | Lincoln Memorial |
1:08.21 |
| 13 | Heath, Abby | JR | Wingate |
1:09.15 |
| 14 | Lay, TraVon | FR | Southern Wesleyan |
1:09.24 |
| 15 | Bailey, Morgan | SO | Carson-Newman |
1:09.47 |
| 16 | Arnold, Briley | JR | North Greenville |
1:09.54 |
| 17 | Rose, Katlyn | FR | North Greenville |
1:11.50 |
| 18 | Warren, Layla | SO | Newberry |
1:15.24 |
| 19 | D'Alesio, Danielle | SO | Carson-Newman |
1:16.26 |
| 20 | Connelly, Samantha | JR | Cumberlands |
1:22.66 |
| 21 | Warren, Nevaeh | FR | Cumberlands |
NT |
| 22 | Siler, Mikkah | FR | Cumberlands |
NT |
| 23 | Lucason, Morgan | FR | Cumberlands |
NT |