| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ALLEN, Jordan | Daniel Boone High School | 59.51 | Verified |
| 2 | HORNE, Joshua | Science Hill High School | 57.77 | Verified |
| 3 | KITZMILLER, Alex | Dobyns Bennett High School | 55.33 | Verified |
| 4 | CARTER, Clay | Dobyns Bennett High School | 54.00 | Unverified: |
| 5 | GILLIAM, Bryson | Dobyns Bennett High School | 52.37 | Verified |
| 6 | LIPFORD, Hayden | Dobyns Bennett High School | 52.52 | Verified |
| 7 | ROSS, John | Volunteer High School | 55.15 | Verified |
| 8 | COX, Cayden | Volunteer High School | 57.25 | Verified |
| 9 | HAGEMEIER, William | Science Hill High School | 58.40 | Verified |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GUILLEN, Juan | Lakeway Christian Academy | NT | Unverified: |
| 2 | SILVERS, Austin | David Crockett High School | NT | Unverified: |
| 3 | FUGATE, Evan | David Crockett High School | 1:04.45 | Verified |
| 4 | WATSON, Emmett | Science Hill High School | NT | |
| 5 | DAVIS, Austin | David Crockett High School | 1:00.55 | Verified |
| 6 | MCGRIFFIN, Palmer | Lakeway Christian Academy | 1:02.46 | Verified |
| 7 | BOWLING, Kole | Volunteer High School | 1:04.33 | Verified |
| 8 | STROUB, Jared | David Crockett High School | NT | Unverified: |
| 9 | DICKERSON, Andrew | Volunteer High School | NT | Unverified: |