| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | David Crockett High School | A | NT | Unverified: |
| 3 | Volunteer High School | A | 49.93 | Verified |
| 4 | Daniel Boone High School | A | 48.74 | Verified |
| 5 | Science Hill High School | B | DQ | Verified |
| 6 | Science Hill High School | A | 45.98 | Verified |
| 7 | Lakeway Christian Academy | A | 49.28 | Verified |
| 8 | Dobyns Bennett High School | A | DNS | Unverified: |
| 9 | Unicoi Co. High School | A | DNS | Unverified: |