| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Anderson (S.C.) |
A | Cannon | Norman | Elson | Clark | NT |
| 4 | Newberry |
A | Elmore | Collins | Grant | Henderson | 43.76 |
| 5 | Queens (N.C.) |
A | Ramos | Payeur | Schittenhelm | Sessions | 46.38 |
| 6 | Wingate |
A | Lepkowski | Holman | Kramer | Jennings | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Catawba |
A | Howard | Walter | Pittman | Noble | 41.75 |
| 3 | Carson-Newman |
A | Dudley | Melancon | Weasby | Moore | 41.36 |
| 4 | Lenoir-Rhyne |
A | Lotharp | Davis | Besong | Turner Jr | DNS |
| 5 | Limestone |
A | Clark | Twitty | Michael | Hart | 40.77 |
| 6 | Coker |
A | Sebree | Greene | Persen | German | 41.72 |
| 7 | Tusculum |
A | Hearst | Purser | Hart | Dupuy | 42.24 |