| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HEDDEN, Abby | Andrews | 28.74 | Verified |
| 2 | ESPINAL, Marley | Hayesville | 28.57 | Unverified: |
| 3 | REVIS, Faith Ann | Murphy | 28.00 | Verified |
| 4 | WEAVER, Ashlyn | Hiwassee Dam | 27.14 | Verified |
| 5 | NELSON, Grace | Murphy | 27.17 | Verified |
| 6 | PARTIN, Rebekah | Rosman | 28.57 | Verified |
| 7 | HELPMAN, Mazie | Swain County | 28.65 | Verified |
| 8 | RODD, Alyssa | Hayesville | 28.85 | Unverified: |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GUERRERO, Kia | Murphy | 30.00 | Unverified: |
| 2 | ANDERSON, Kiara | Hiwassee Dam | 29.94 | Verified |
| 3 | COOLEY, Lili | Enka | 29.89 | Verified |
| 4 | JACKSON, Sierra | Swain County | 29.74 | Verified |
| 5 | RAPER, Tiffany | Hiwassee Dam | 29.84 | Verified |
| 6 | PHILLIPS, Brooke | Andrews | 29.94 | Verified |
| 7 | LEDFORD, Christina | Hayesville | 29.99 | Unverified: |
| 8 | ADAMS, Dezeray | Hiwassee Dam | 30.10 | Unverified: Practice |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MANUEL, Erin | Tri-County Early College | 31.44 | Verified |
| 2 | ESCOBAR, Dalia | Tri-County Early College | 31.27 | Verified |
| 3 | MCGUIRE, Chelsea | Franklin | 30.94 | Verified |
| 4 | BURRELL, Corey | Franklin | 30.24 | Verified |
| 5 | ANSARI, Jaida | Rosman | 30.48 | Verified |
| 6 | GALLOWAY, Maggi | Rosman | 30.94 | Verified |
| 7 | ADJAIDOO, keisha | Enka | 31.34 | Verified |
| 8 | BARREIRO, Vivianna | Murphy | 32.00 | Unverified: |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | FRADY, Joise | Enka | 37.20 | Unverified: |
| 2 | BASHAM, Lauren | Franklin | 33.53 | Verified |
| 3 | ROBERTS, Kalyn | Andrews | 32.44 | Verified |
| 4 | NAPRYIENKO, Adelina | Enka | 32.00 | Unverified: |
| 5 | SCHEU, Annelise | Hayesville | 32.00 | Unverified: |
| 6 | MCLEOD, Abby | Swain County | 33.18 | Verified |
| 7 | BRADFORD, Zoe | Franklin | 35.00 | Unverified: |
| 8 | SOTO, Nicole | North Carolina School for the De | 47.36 | Verified |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | LILLARD, Tynighia | Swain County | NT | Unverified: |
| 3 | BRYSON, Beatrice | Highlands | NT | Unverified: |
| 4 | BUCHANAN, Laikyn | Rosman | NT | Unverified: |
| 5 | KING, Dalericka | Cherokee | NT | Unverified: |
| 6 | BRANHAM, Bella | Highlands | NT | Unverified: |