| Lane | Athlete | Age | Team | Seed | Note | 
|---|---|---|---|---|---|
| 2 | Gregory, Trinyty | 16 | Lane 4 Track Club | 1:03.33 | |
| 3 | Crustrini, Alice | 14 | Elite Track and Field Traini | 59.00 | |
| 4 | Lucas, Olicia | 15 | Elite Track and Field Traini | 56.50 | |
| 5 | Walker, Keniya | 16 | Unattached | 58.30 | |
| 6 | Forte, Sanai' | 14 | Purpose Driven NC Elite Trac | 1:00.00 | |
| 7 | Jones, Phiasia | 15 | The Storm Track Club | 1:04.06 | 
| Lane | Athlete | Age | Team | Seed | Note | 
|---|---|---|---|---|---|
| 3 | Saulters, Sidnee | 14 | Unattached | NT | |
| 4 | Kammer, Allison | 14 | Mt. Pleasant Track Club | 1:06.44 | |
| 5 | Thompson, Bryanna | 15 | Fast Forward Track Club | 1:07.00 |