| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | Williams, Charity | 18 | Unattached | NT | |
| 3 | Presley, T'Kye | 17 | Elite Track and Field Traini | 1:04.00 | |
| 4 | Bellamy, Shani'a | 18 | Unattached | 1:01.97 | |
| 5 | Gordon, Lauren | 18 | Unattached | 1:03.01 | |
| 6 | Diaz, Nicole | 18 | Purpose Driven NC Elite Trac | 1:22.86 |