| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Bryant, Justin | 14 | The All American Project | 56.90 | |
| 2 | Brown, Donnovan | 16 | Unattached | 54.04 | |
| 3 | Chapman, Philip | 16 | The Storm Track Club | 53.11 | |
| 4 | Parker, Christian | 15 | The All American Project | 49.80 | |
| 5 | Dawes, Elijah | 15 | The All American Project | 49.80 | |
| 6 | Threatt, Keenan | 15 | Elite Track and Field Traini | 54.00 | |
| 7 | McCovery, Langston | 16 | Lane 4 Track Club | 55.67 |