| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | Moyer, Trevin | 16 | Unattached | 49.50 | |
| 4 | Brewer Jr, Robert | 17 | Unattached | 48.95 | |
| 6 | Leacock, Dillon | 17 | Unattached | 49.11 | |
| 8 | Baratta, Anthony | 17 | Unattached | 51.00 |
| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Beard, Leonardo | 16 | Lane 4 Track Club | 53.37 | |
| 3 | White, Eric | 19 | Lane 4 Track Club | 51.37 | |
| 5 | Coleman, Christopher | 15 | Need 4 Speed | 52.20 | |
| 7 | Jones, Vance | 16 | Unattached | 55.00 |
| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | Clary, Kymani | 11 | Mt. Pleasant Track Club | 1:08.44 | |
| 4 | Edwards, Jericho | 12 | Winston Salem Roadrunners | 1:01.50 | |
| 6 | Granville, Deaven | 13 | Lane 4 Track Club | 1:04.07 |
| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | Tonelis, Miles | 9 | Mt. Pleasant Track Club | NT | |
| 5 | Mills, George | 8 | Lane 4 Track Club | 1:13.33 | |
| 7 | Henry, Bryson | 6 | Unattached | 1:25.00 |