| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | KENYERES, Valentina | Milligan |
2:23.62 | LRU Southside Power & Fitness Invitational |
| 2 | ALLEN, Gracie | Milligan |
2:19.00 | |
| 3 | RISH, Olivia | Anderson (S.C.) |
2:16.00 | |
| 4 | QUARTARARO, Brianna | Anderson (S.C.) |
2:21.60 | USC Indoor Open |
| 5 | WARD, Rebecca | Anderson (S.C.) |
2:24.00 | |
| 6 | BLADEN, Autumn | Anderson (S.C.) |
2:25.83 | South Atlantic Indoor Track & Field Championships |
| 7 | OSTERBIND, Kamryn | Anderson (S.C.) |
2:16.00 | |
| 8 | CHILDERS, Lauren | Anderson (S.C.) |
2:24.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PERNELL, Lauren | Anderson (S.C.) |
2:35.00 | |
| 2 | GLOVER, Rebecca | Milligan |
2:39.40 | ETSU Track & Field Invitational 2020 |
| 3 | LANDRY, Katie | Anderson (S.C.) |
2:30.00 | |
| 4 | HAYDEN, Joeli | Anderson (S.C.) |
2:35.00 | |
| 5 | DUTTON, Morgan | Erskine |
NT | |
| 6 | EKUNDAYO, Esther | Columbia (S.C.) |
2:32.31 | Appalachian AC Indoor Track & Field Championships |
| 7 | ARDIS, Natalie | Anderson (S.C.) |
2:50.00 | |
| 8 | CLARY, Kaliyah | Fast Forward Track Club | 2:30.00 | |
| 9 | THOMPSON, Katherine | Erskine |
NT |