| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | CREECH, CARI | JR | Sullivan South High School | 1:04.67 | Verified |
| 3 | FARLEY, CASSIDY | JR | Abingdon High School | 1:03.74 | Verified |
| 4 | GLOVER, REBECCA | SR | Sullivan East High School | 1:01.71 | Verified |
| 5 | CORNETT, SARAH | JR | Patrick Henry (G. Spring) | 1:00.81 | Verified |
| 6 | ARNOLD, EMMA | JR | Tennessee High School | 1:01.23 | Verified |
| 7 | CHRISTIAN, MEGAN | JR | Volunteer High School | 1:01.82 | Verified |
| 8 | SCOTT, HALLE | SO | David Crockett High School | 1:04.20 | Verified |
| 9 | MULLINS, KATE | SR | Abingdon High School | 1:06.38 | Verified |