| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Daniel Boone High School | A | 10:11.24 | Verified | ||||
| 2 | Volunteer High School | A | 10:58.92 | Verified | ||||
| 3 | Castlewood | A | 11:05.16 | Verified | ||||
| 4 | David Crockett High School | A | Dulaney, Rachel | Hulse, Emmaline | Scott, Halle | Roy, Taylor | 11:05.92 | Unverified: Science Hill Varsity Meet #9 |
| 5 | University School of Johnson Cit | A | Johnson, Isabelle | Day, Kirsten | Bader, Anna | Burns, Ali | 11:09.01 | Verified |
| 6 | Happy Valley High School | A | Miller, Haley J. | Moore, Holly | Orfield, Holly | Simerly, Tatiana | 12:14.07 | Verified |
| 7 | West Greene High School | A | 13:18.98 | Verified |