| World | 3:23.37 | Gushchina, Kotlyarova, Zaytseva, Krasnomovets | RUS | ||
| American | 3:27.34 | Dunn, Trotter, Hastings, Felix | U.S.A. | ||
| American HS | 3:38.91 | Poly, L. Beach, CA | |||
| ! | USA # 1 | 3:46.29 | |||
| @ | USA #10 | 4:00.08 | |||
| * | USA #20 | 4:03.20 | |||
| NCAA (banked) | 3:34.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | East Tenn. St. | A | Smith, Nicki | Simmons, Lamisha | Davis, Kiana | Lord, Jessica | 3:54.04 | |
| 2 | Southern Wesleyan | B | Campbell, Chyna | Julian, Skylar | Williams, Keyanna | Massey, Destiny | 3:47.06 | 2017 Aggie Last Chance |
| 3 | Western Carolina | A | Whitehead, Kellsie | Woerner, Sally | Hampton, Ty-Leah | Arnaut, Dasha | 3:45.30 | 2016 Southern Conference Outdoor T&F Championships |
| 4 | Southern Wesleyan | A | Barton, Amy | King, Lyric | Achenbach, Kayla | Lewis, Jami | 3:47.06 | 2017 Aggie Last Chance |
| 5 | Wofford | A | Dean, Laura | Morton, Kassidy | Combo, LeAndrea | Head, Olivia | 3:49.80 | Southern Conference Outdoor Championships 2017 |
| 6 | Appalachian State | A | Chapman, Imani | Dixon, Jessica | Gibson, Samara | Morrison, Erin | 3:50.66 | Sun Belt Conference Outdoor Track & Field Championships |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lenoir-Rhyne | B | Drummonds, Savannah | Jones, Mya | Medley, Leona | Meeks, Summer | 4:01.45 | UNCG Early Bird Duals |
| 2 | East Tenn. St. | B | Graham, Starr | Atkins, Kyra | Lacy, Jazmin | Sanoe, Fanta | DNF | |
| 4 | High Point | A | Gillespie, Jasmyn | Soper, CeAnna | Weisbrod, Anika | Reynolds, Madison | 3:57.99 | Big South Indoor Track & Field Championships |
| 5 | Lenoir-Rhyne | A | Fair, Paiton | Hardwick, Saidah | Alston, Dakota | McElveen, Keyua | 4:01.45 | UNCG Early Bird Duals |
| 6 | Tennessee Wesleyan | A | Hooijdonk, Els | Lankford, Alexis | Condone, Bailey | Reviere, Malika | DNS | Appalachian Athletic Conference Championship |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Tusculum | B | Blackwood, Autumn | Lewis, Jayla | McDonald , Briana | Wildman, Drew | 4:35.28 | JDL College Kick-off Classic |
| 2 | Lees-McRae | A | McCoy, Millie | Gearing, Kaitlyn | Estep, Makayla | Goras, Rebeckah | 4:28.28 | ETSU Track & Field Invitational |
| 3 | King | A | Graham, Rachel | McCormick, Audrey | Pierce, Abbigail | Rivera, Kaitlin | 4:12.64 | 2016 Conference Carolinas Championships |
| 4 | Guilford | A | Cooper, Chantell | Newkirk, A'lexus | Scott, Kayla | Wright, Cheyenne | 4:27.97 | ODAC Outdoor Track and Field Championships |
| 5 | Tusculum | A | Pruiett, Bailey | Minus, Raygene | Griffith, Kesia | Cole, Hailey | 4:35.28 | JDL College Kick-off Classic |
| 6 | Lincoln Memorial | A | NT |