| PL | # | Athlete | Team | Time | HPL |
|---|---|---|---|---|---|
| 1 | 155 | RODRIGUEZ, Pedro | MIAMI ELITE TRACK CLUB | 43.58 | 3 (1) |
| 2 | 320 | CROSSMAN, Colin | VISION AND TRUST TC | 44.19 | 2 (1) |
| 3 | 175 | CHIN, Jadon | STRIDE STARS | 45.66 | 3 (2) |
| 4 | 208 | SAINT-FLEUR, Amos | SWIFFERS TRACK CLUB | 46.05 | 1 (1) |
| 5 | 138 | DUQUE, Salvador | MIAMI ELITE TRACK CLUB | 47.76 | 3 (3) |
| 6 | 176 | TELCINE, Jaden | STRIDE STARS | 48.04 | 3 (4) |
| 7 | 156 | RODRIGUEZ ZARATE, Socrates | MIAMI ELITE TRACK CLUB | 48.78 | 2 (2) |
| 8 | 209 | TAYLOR, Micah | SWIFFERS TRACK CLUB | 50.47 | 3 (5) |
| 9 | 140 | INHOOF, Jordan | MIAMI ELITE TRACK CLUB | 51.25 | 1 (2) |
| 10 | 168 | YOUNG-SANG, Raiden | STRIDE STARS | 52.11 | 2 (3) |
| 11 | 139 | GUTIERREZ, Adrian | MIAMI ELITE TRACK CLUB | 52.32 | 2 (4) |
| 12 | 166 | COSTA, Eli | STRIDE STARS | 53.28 | 2 (5) |
| 13 | 100 | CAMILO, Derrick | GOULDS PANTHERS TRACK CLUB | 55.00 | 1 (3) |
| 14 | 169 | YOUNG-SANG, Raizo | STRIDE STARS | 56.52 | 2 (6) |
| 15 | 210 | VOLTAIRE, Judah | SWIFFERS TRACK CLUB | 58.25 | 3 (6) |