| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Cedarville |
A | Brizzi | Franklin | Musick | Hagan | DNS |
| 2 | Tiffin |
A | Strauther | Janis | Richardson | Andrews | 47.41 |
| 3 | Walsh |
A | Hartsock | Henry | Martin | Epps | 46.90 |
| 4 | Findlay |
A | Fishpaw | Ivory | Ray | Taylor | 46.51 |
| 5 | Ashland |
A | Dennis | Williams | Barker | Wojton | 46.63 |
| 6 | Tiffin |
B | Arroyo | Roby | Culler | Rembert | 47.41 |
| 7 | John Carroll |
A | Anthony | Arnold | Scott | Murray | 47.84 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Ohio Dominican |
A | Owens | Morrison | Ambos | Van Horn | DNS |
| 4 | Baldwin Wallace |
A | Hurd | Aloi | Bradley | Collier | 50.27 |
| 5 | Malone |
A | Reifsnyder | Morris | Farrell | Hicks | 51.95 |