| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MACADAM, Ines | Tiffin |
5:30.00 |
| 2 | JAREMA, Courtney | Oakland |
5:25.00 |
| 3 | GRINDLING, Nicole | Oakland |
5:20.13 |
| 4 | SPRINGER, Julia | Tiffin |
5:53.45 |
| 5 | LINDSEY, Ashlin | Tiffin |
6:04.85 |
| 6 | PAPADELIS, Marisa | Oakland |
5:38.00 |
| 7 | MARKEL, Morgan | Oakland |
5:20.00 |
| 8 | NIBITANGA, Esperance | Kentucky State |
6:00.00 |
| 9 | WYANT, Ruthie | Tiffin |
6:14.24 |
| 10 | IFFLAND, Jordan | Tiffin |
NT |
| 11 | UPDIKE, Olivia | Oakland |
5:35.00 |
| 12 | CALDWELL, Carmen | Defiance |
NT |
| 13 | GUGGENBILLER, Audrey | Tiffin |
NT |