| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Freedom (Tampa) |
A | Smith | Haywood | Thomas | Lambert | 44.65 |
| 3 | Wharton |
B | Cordoba | Hicks | Hilton | Howard | 41.92 |
| 4 | Wharton |
A | Abimbola | Blackmon | Brown | Butler | 41.92 |
| 5 | Wharton |
D | Rodriguez | Smith | Staten | Stratford | 41.92 |
| 6 | Wharton |
C | Marcelin | Martinez | Montgomery | Nesmith | 41.92 |