| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Carrollwood | B | Dudley | Holmes | Hussey | Johnson | NT |
| 4 | Tampa Catholic | B | Dimaria | Pompilus | Ware | Evans | NT |
| 5 | Carrollwood | A | Brown | Burney | Siemiet | Williams | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Blake | B | Byrd | Mobley | Collins | Jackman | 46.66 |
| 2 | Jefferson | A | Turner | Gunn | Bacon | Young | NT |
| 3 | Tampa Catholic | A | Carter | Moore | Mitchell | Grimsley | 44.13 |
| 4 | Wharton | A | Christmas | Littleton | Jackson | Greene | 43.08 |
| 5 | Wharton | B | Mims | Hineline | Chapper | Smith | NT |
| 6 | Jefferson | B | Jenkins | Morton | Russell | Kalinin | 44.73 |
| 7 | Blake | A | Haynes | Collins | Colbert | Kanu | 46.66 |