| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Chasco Middle School | A | Mason | Scorca | Doty | Pantin-Henriquez | NT |
| 3 | Seven Springs Middle School | B | Tifer | Jaye | Bronson | Casarez | 4:45.00 |
| 4 | Seven Springs Middle School | A | York | Thomas | Blumenstein | Glennon | 4:25.00 |
| 5 | Gulf Middle School | A | Sannoh | Starkey | Marquis | Brown | 4:27.59 |
| 6 | Bayonet Point Middle School | A | Brinson | Iovino | Robinson | Rodriguez | NT |