| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Leto |
A | Santiago | Adams | Grabarski | White | 47.03 |
| 3 | King |
A | Callaway | Louissant | Hobbs | Reid | 43.84 |
| 4 | Tampa Bay Tech |
A | Zayas | Houston | Floyd | Daniel | 41.09 |
| 5 | Blake |
A | Lucas | Shaw | Smith-Moore | Lormera | 43.60 |
| 6 | Middleton |
A | Hardy | Prassanna | Stanford | Lawrence | 45.21 |