| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Brandon | B | AMBROCIO | Mendez | Peoples | Shine | NT | 
| 3 | Sumner | B | Hudson | Walker | Williams | Clarke | 42.37 | 
| 4 | Newsome | B | Dempsey | Lesh | Holt | Taylor | 42.11 | 
| 5 | Newsome | A | Pflasterer | Roque | Soto | Connally | 42.11 | 
| 6 | Sumner | A | Bruce | Taylor | Moss, III | Hendricks | 42.37 | 
| 7 | Brandon | A | RENDER | Charles | Colbert | Shine | 48.37 |