| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Mitchell | B | Maxton | Cletcher | Erwin | Minaca | NT |
| 4 | Wesley Chapel | B | Rodriguez | Kirkwood | Schmidt | Scott | 4:18.08 |
| 5 | Mitchell | A | Alejandro-Rivera | Cain | D'Piazza | Christiansen | NT |
| 6 | Sunlake | A | Culpepper | Spires | Newport | Viseur | 4:21.29 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Wiregrass Ranch | A | Khalil | Fleming | Claunch | Potter | NT |
| 4 | Land O'Lakes | B | Giesler | Connoy | Christie | Farrell | 4:14.70 |
| 5 | Land O'Lakes | A | Denney | Denney | Johnson | Cole | 4:14.70 |
| 6 | Wesley Chapel | A | Vaile | Harris | Rogers | Dove | 4:18.08 |
| 7 | Sunlake | B | Smith | Antunez | Christy | Epps | 4:21.29 |