| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Tampa Bay Tech | B | Boone | Lundy | Bethune | Hannor | NT | 
| 3 | Tampa Bay Tech | A | Joseph | Gassett | Boone | Roque-Hernandez | 3:45.00 | 
| 4 | Robinson | A | Johnson | Cochran | Difo | Pham | 3:38.15 | 
| 5 | Gaither | A | Demps | Wilson | Summers | Gardiner | 3:42.00 | 
| 6 | Gaither | B | Ballard | Payne | Martinez | Golkar | NT |