| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Riverview |
B | Holloman | Lugo | Robinson | Luna | 43.67 |
| 2 | Strawberry Crest |
B | Padilla | Brown | Harris | Nguyen | 43.36 |
| 3 | East Bay |
B | Cooper | Brown | Simms | Williams | 43.22 |
| 4 | Armwood |
A | Adams | Hudgins | Jones | Clark | 41.22 |
| 5 | East Bay |
A | Alexander | Koonce | Person | McClary | 43.22 |
| 6 | Strawberry Crest |
A | Brantley | Brown | Lawson | Martinez-Ramos | 43.36 |
| 7 | Riverview |
A | Bailey | Tatum-Lesane | Booker | Holliday | 43.67 |
| 8 | Bell Creek |
A | Cenozier | Mireles | Johnston | Guild | 47.92 |