| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | LOCKNER, Sydney | Newsome | 2:31.00 |
| 2 | WELLS, Megan | Newsome | 2:32.00 |
| 3 | SHAH, Anika | Newsome | 2:34.00 |
| 4 | COLEMAN, Zaria | Plant City | 2:50.00 |
| 5 | SHAFFER, Rylee | Plant City | 2:50.00 |
| 6 | STEPHENS, Haillie | Newsome | 2:50.50 |
| 7 | BRICKHAUS, Jessica | Newsome | 2:55.00 |
| 8 | CANNON, Mary | Plant City | 2:55.00 |
| 9 | HEADLEY, Destiny | Plant City | 2:55.00 |
| 10 | JACOBSON, Ashlynn | Newsome | 2:59.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | LANDEZ, Avery | Newsome | 3:00.00 |
| 2 | CRISPIN, Jacqueline | Plant City | 3:05.00 |
| 3 | MAIDENS, Jocelyn | Newsome | 3:05.00 |
| 4 | CASTRO, Johanna | Riverview | 3:08.00 |
| 5 | CONNELL, Kenley | Plant City | 3:20.00 |
| 6 | GUERRERO, Sophia | Riverview | 3:33.00 |
| 7 | KROPP, Kiana | Plant City | NT |