| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Chamberlain |
A | Urquhart | Altman | Brim | Sweet | NT |
| 3 | Plant |
A | Boothe | Bowman | Bryce-Wells | Craig | 51.88 |
| 4 | Hillsborough |
A | Wiley | Miller | Miller | Scott | 50.35 |
| 5 | Alonso |
A | Urbano-Umbacia | Desmond | Zhou | Kilkelly | 50.41 |
| 6 | George Steinbrenner |
A | Colon | Skeins | Duran | Patterson | NT |