| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | BOGAN, Xavier | JR | Hillsborough | 52.08 |
| 2 | JOHNSON, Trayvun | SR | King | 51.21 |
| 3 | JOHNSON, Lakeem | JR | Robinson | 50.99 |
| 4 | STIFF, Amir | JR | Tampa Bay Tech | 50.75 |
| 5 | SAPP, Tredarius | SR | Newsome | 50.90 |
| 6 | OLIVER, DaJohn | SR | Middleton | 51.11 |
| 7 | PIERRE, Masqal | SR | Gaither | 51.71 |
| 8 | BROWN, Keashawn | SR | Tampa Bay Tech | 52.30 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | YOUNG, Jaiden | SR | Jefferson | 50.25 |
| 2 | THOMPSON, J.T. | SR | Sickles | 49.72 |
| 3 | TUCKER, Mychael | SR | East Bay | 49.11 |
| 4 | CAMPBELL, Camren | JR | Tampa Bay Tech | 48.46 |
| 5 | MORRIS JR, Dontae | JR | Tampa Bay Tech | 48.48 |
| 6 | SHOWERS, Aiden | SR | East Bay | 49.20 |
| 7 | BROWN, Sean | SR | Hillsborough | 49.74 |
| 8 | MCCLENDON, Joshua | SR | Sumner | 50.70 |